Regulatory
Part:BBa_K337039
Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R Group: iGEM10_Heidelberg (2010-10-22)
TetR_mut(XhoI/XbaI)
Mutated TetR where XhoI/XbaI sites were introduced right after the stop codon enabling easy introduction of synthetic microRNA binding sites into the 3'UTR of the TetR (BB-2, RFC12).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 10
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 652
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 10
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 10
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |